Journal: Cell Reports

Article Title: Pressure and curvature control of the cell cycle in epithelia growing under spherical confinement

doi: 10.1016/j.celrep.2022.111227

Figure Lengend Snippet:

Article Snippet: 3D printer resin , EnvisionTEC , HTM140V2.

Techniques: Recombinant, Protease Inhibitor, Software, Microscopy, Membrane

Journal: Cell Reports

Article Title: Pressure and curvature control of the cell cycle in epithelia growing under spherical confinement

doi: 10.1016/j.celrep.2022.111227

Figure Lengend Snippet:

Article Snippet: The microfluidic device (MD) was printed with the 3D printer EnvisionTEC Micro Hi-Res Plus, using the resin HTM140V2 (EnvisionTEC), with expected Z resolution of 25μm.

Techniques: Recombinant, Protease Inhibitor, Software, Microscopy

Journal: bioRxiv

Article Title: Buckling of epithelium growing under spherical confinement

doi: 10.1101/513119

Figure Lengend Snippet: a , 3D-printed microfluidic device. b , draft of the side view of the microfluidic device. The green, blue and yellow colors represent alginate, sorbitol solution and cell solution flows, respectively. c , draft of the back view of the microfluidic device. The green, blue and yellow colors represent alginate, sorbitol solution and cell solution flows, respectively. d , a photo of the experimental setup. e , confocal equatorial plane of a part of the capsule (green) showing the layer of Matrigel (red) with Cy3-labeled Laminin. f , Phase-contrast microscope image of 2.5% alginate capsule with growing epithelial monolayers, at 24h after capsule formation. g , Box-plot of mean capsule radius as a function of alginate solution percentage.

Article Snippet: The microfluidic device was printed with EnvisionTEC Micro Hi-Res Plus 3D printer using the resin HTM140V2 (EnvisionTEC), with the following printing parameters (set automatically based on the resin used): burn-in range thickness 400 μm, base plate of 300 μm, and exposure time 3000 ms.

Techniques: Labeling, Microscopy